James Christopher Wright ([info]bifo) wrote,
@ 2007-05-21 14:23:00
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Current location:Dover St, Manchester, UK
Current mood: cheerful
Entry tags:consensus spectra, creatine kinase, electrophoresis, pmf, profiles, zoo gels

PMF Consensus Profiles For Cross Species Proteomics
Well after collecting MALDI-TOF spectra last week from my "Zoo Gels" (1d SDS-PAGE Gel electrophoresis of "Rat", "Chinchilla", "Vole", "Pig", "Sheep", and "Goat" muscle protein samples) I have begun compiling the results into consensus spectra.

Looks like I can only really do it for "Creatine Kinase" at this stage because I didn't get many Mascot protein identifications, mainly because there are not many proteins sequenced for most of these species and that the samples where quite old and I'm not very sure that the spectra are of any usable quality.

Anyway it does seem to work which could mean that I will be over in Liverpool collecting lots of Peptide Mass Fingerprint data in the near future.



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